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Direct analysis in real-time (DART) time-of-flight mass spectrometry (TOFMS) of wood reveals distinct chemical signatures of two species of Afzelia / Peter Kitin in Annals of Forest Science, vol 78 n° 2 (June 2021)  

Public [article]  inAnnals of Forest Science > vol 78 n° 2 (June 2021) . - Article 31 Titre : Direct analysis in real-time (DART) time-of-flight mass spectrometry (TOFMS) of wood reveals distinct chemical signatures of two species of Afzelia Type de document : Article/Communication Auteurs : Peter Kitin, Auteur ; Edgard Espinoza, Auteur ; Hans Beeckman, Auteur ; et al., Auteur Année de publication : 2021 Article en page(s) : Article 31 Note générale : Bibliographie Langues : Anglais (eng) Descripteur : [Vedettes matières IGN] Traitement d'image [Termes IGN] abattage (sylviculture) [Termes IGN] Afzelia (genre) [Termes IGN] analyse discriminante [Termes IGN] apprentissage non-dirigé [Termes IGN] bois [Termes IGN] espèce végétale [Termes IGN] forêt tropicale [Termes IGN] identification de plantes [Termes IGN] signature spectrale [Termes IGN] spectrométrie [Termes IGN] taxinomie [Termes IGN] temps réel Résumé : (Auteur) Distinct chemical fingerprints of the wood of Afzelia pachyloba and A. bipindensis demonstrated an effective method for identifying these two commercially important species. Direct analysis in real-time (DART) time-of-flight mass spectrometry (TOFMS) allowed high-throughput examination of chemotypes with vast potential in taxonomic, ecological, and forensic research of wood. Context : Afzelia is a genus of valuable tropical timber trees. Accurate identification of wood is required for the prevention of illicit timber trade as well as for certification purposes in the forest and wood products industry. For many years, particular interest has been focused on attempts to distinguish the wood of A. bipindensis Harms from A. pachyloba Harms due to substantial differences in the commercial values of these two species. Aims : We investigated if wood chemical signatures and microscopy could identify the wood of A. bipindensis and A. pachyloba. Methods : We used two approaches, namely metabolome profiling by direct analysis in real-time (DART) time-of-flight mass spectrometry (TOFMS) and wood microstructure by light microscopy and SEM. In all, we analyzed samples from 89 trees of A. bipindensis, and A. pachyloba. Results : The two species could not be separated by the IAWA standard microscopic wood features. SEM analysis showed considerable variation in the morphology of vestured pits; however, this variation was not species-specific. In contrast, DART-TOFMS followed by unsupervised statistics (Discriminant Analysis of Principal Components) showed distinct metabolome signatures of the two species. Conclusion : DART-TOFMS provides a rapid method for wood identification that can be easily applied to small heartwood samples. Time- and cost-effective classification of wood chemotypes by DART-TOFMS can have potential applications in various research questions in forestry, wood science, tree-ecophysiology, and forensics. Numéro de notice : A2021-327 Affiliation des auteurs : non IGN Thématique : FORET/IMAGERIE Nature : Article nature-HAL : ArtAvecCL-RevueIntern DOI : 10.1007/s13595-020-01024-1 Date de publication en ligne : 31/03/2021 En ligne : https://doi.org/10.1007/s13595-020-01024-1 Format de la ressource électronique : URL article Permalink : https://documentation.ensg.eu/index.php?lvl=notice_display&id=97488 [article]