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Biochemical peculiarity of in vitro morphogenesis under conservation strategy of Ruscus aculeatus L. / Cristian Banciu in Annals of forest research, vol 52 n° 1 (January 2009)    

Public [article]  inAnnals of forest research > vol 52 n° 1 (January 2009) . - p. 109-115 Titre : Biochemical peculiarity of in vitro morphogenesis under conservation strategy of Ruscus aculeatus L. Type de document : Article/Communication Auteurs : Cristian Banciu, Auteur ; Monica Elena Mitoi, Auteur ; Aurelia Brezeanu, Auteur Année de publication : 2009 Article en page(s) : p. 109-115 Note générale : Bibliogr. Langues : Anglais (eng) Descripteur : [Termes IGN] conservation de la flore [Termes IGN] microbouturage [Termes IGN] morphogenèse [Termes IGN] Ruscus aculeatus Résumé : (Auteur) "The present study is part of the actual concerns in biodiversity conservation of endangered angyosperm species from the Comana Natural Park. Ruscus aculeatus L. species is protected both at national and European level (trough Habitats Directive of EU and Bern Convention). The aspects of in vitro morphogenesis through all the stages from inoculation, multiplication to rooting and acclimatization have been studied. In order to long term conservation and multiplication or to exploit somaclonal variation induced by in vitro technique is required the identification of some biochemical or molecular markers for fast evaluation of regenerants. In this respect, genetic stability or variability of Ruscus aculeatus plants collected from three populations and regenerants obtained in vitro culture was estimated by electrophoretical methods. Therefore, zymograms of different enzymes as esterase, acid and alkaline phosphatase, glutamate-oxaloacetate transferase, malate dehydrogenase and peroxidase were analyzed. The expression of esterases, phosphatases, malate dehydrogenases displayed changes in correlation with growth condition, while the peroxidases pattern was more stabile in natural population as well as in vitro regenerated plantlet." Numéro de notice : IFN_5799 Affiliation des auteurs : non IGN Thématique : BIODIVERSITE Nature : Article DOI : sans En ligne : http://www.editurasilvica.ro/afr/52/1/10_Banciu.pdf Permalink : https://documentation.ensg.eu/index.php?lvl=notice_display&id=73511 [article]

Documents numériques

en open access Biochemical peculiarity of in vitro morphogenesis... Adobe Acrobat PDF

Conservarea ex situ a resurselor genetice de plop negru (Populus nigra L.) utilizând culturile un vitro / Lucia Ionita in Revista Padurilor, vol 124 n° 1 (1/2009)

Public [article]  inRevista Padurilor > vol 124 n° 1 (1/2009) . - p. 5-9 Titre : Conservarea ex situ a resurselor genetice de plop negru (Populus nigra L.) utilizând culturile un vitro Titre original : Ex situ preservation of black poplar (Populus nigra L.) genetic resources using in vitro volume = Conservation ex situ des ressources génétiques du peuplier noir (Populus nigra L.) à l'aide des cultures in vitro Type de document : Article/Communication Auteurs : Lucia Ionita, Auteur Année de publication : 2009 Article en page(s) : p. 5-9 Note générale : Résumé en anglais Langues : Roumain (rum) Descripteur : [Termes IGN] biotechnologie [Termes IGN] génétique forestière [Termes IGN] microbouturage [Termes IGN] Populus nigra Numéro de notice : IFN_4893 Affiliation des auteurs : non IGN Nature : Article DOI : sans Permalink : https://documentation.ensg.eu/index.php?lvl=notice_display&id=72954 [article]

Exemplaires(1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
IFN-001-P000717	PER	Revue	Nogent-sur-Vernisson	Archives périodiques	Exclu du prêt

Influence of microstructured carbon materials on Sequoia sempervirens (D. Don.) Endl. in vitro culture / Raluca Stoiculescu in Annals of forest research, vol 52 n° 1 (January 2009)    

Public [article]  inAnnals of forest research > vol 52 n° 1 (January 2009) . - p. 137-141 Titre : Influence of microstructured carbon materials on Sequoia sempervirens (D. Don.) Endl. in vitro culture Type de document : Article/Communication Auteurs : Raluca Stoiculescu, Auteur ; Gina Cogalniceanu, Auteur ; Aurelia Brezeanu, Auteur ; Gabriela Hristea, Auteur Année de publication : 2009 Article en page(s) : p. 137-141 Note générale : Bibliogr. Langues : Anglais (eng) Descripteur : [Termes IGN] carbone [Termes IGN] microbouturage [Termes IGN] Sequoia sempervirens Résumé : (Auteur) "The present experimental research has been mainly focused on Sequoia sempervirens (D. Don.) Endl. as woody plant model system, for testing the influence of some microstructured carbon materials (CCM) comparing with the activated charcoal action, on the in vitro growth. The work hypothesis was that CCM can induce effects on the plant culture, similar to those induced by activated charcoal, due to their high absorption capacity. The influence of two types CCM and activated charcoal introduced in the medium culture was evaluated on the fresh weight and on the length of the in vitro regenerants, after 30 and 60 days of cultivation. Our data revealed that the CCM is not toxic and does not inhibit plant growth. We also remarked that S. sempervirens (D. Don.) Endl. was more reactive to the activated charcoal into the nutritive medium than the CCM compounds. The use of these new microstructured compounds is an important step both in the study of materials science and in the field of plant biotechnologies." Numéro de notice : IFN_5802 Affiliation des auteurs : non IGN Thématique : FORET Nature : Article DOI : sans En ligne : http://www.editurasilvica.ro/afr/52/1/13_Stoiculescu.pdf Permalink : https://documentation.ensg.eu/index.php?lvl=notice_display&id=73514 [article]

Documents numériques

en open access Influence of microstructured carbon materials on Sequoia sempervirens... Adobe Acrobat PDF

The establishment of an in vitro gene bank in Dianthus spiculifolius Schur and D. glacialis ssp. gelidus (Schott Nym. et Kotschy) Tutin: I. The initiation of a tissue collection and the characterization of the cultures in minimal growth conditions / Mihaela Holobiuc in Annals of forest research, vol 52 n° 1 (January 2009)  

Public [article]  inAnnals of forest research > vol 52 n° 1 (January 2009) . - pp 117 - 128 Titre : The establishment of an in vitro gene bank in Dianthus spiculifolius Schur and D. glacialis ssp. gelidus (Schott Nym. et Kotschy) Tutin: I. The initiation of a tissue collection and the characterization of the cultures in minimal growth conditions Type de document : Article/Communication Auteurs : Mihaela Holobiuc, Auteur ; Rodica Blindu, Auteur ; Monica Mitoi, Auteur ; Florenta Helepciuc, Auteur ; Victoria Cristea, Auteur Année de publication : 2009 Article en page(s) : pp 117 - 128 Note générale : Bibliogr. Langues : Anglais (eng) Descripteur : [Termes IGN] croissance végétale [Termes IGN] Dianthus glacialis [Termes IGN] Dianthus spiculifolius [Termes IGN] diversité génétique [Termes IGN] microbouturage [Termes IGN] politique de conservation (biodiversité) [Vedettes matières IGN] Végétation et changement climatique Résumé : (Auteur) In the last decades, the plants have to cope with the warming of the climate. As a consequence of this process more than half of the plant species could become vulnerable or threatened until 2080. Romania has a high plant diversity, with endemic and endangered plant species, the measures of biodiversity conservation being necessary. The integrated approach of biodiversity conservation involves both in situ and ex situ strategies. Among ex situ methods of conservation, besides the traditional ones (including field and botanic collection and seed banks), in vitro tissues techniques offer a viable alternative. The germplasm collections can efficiently preserve the species (of economic, scientific and conservative importance), in the same time being a source of plant material for international exchanges and for reintroduction in the native habitats.The "in vitro gene banking" term refers to in vitro tissues cultures from many accessions of a target species and involves the collection of plant material from field or from native habitats, the elaboration of sterilization, micropropagation and maintaining protocols. These collections have to be maintained in optimal conditions, morphologically and genetically characterized. The aim of our work was to characterize the response of the plant material to the minimal in vitro growth protocol for medium-term cultures achievement as a prerequisite condition for an active gene bank establishment in two rare Caryophyllaceae taxa: Dianthus spiculifolius and D. glacialis ssp. gelidus. Among different factors previously tested for medium-term preservation in Dianthus genus, mannitol proved to be more efficient for minimal cultures achievement. In vitro, the cultures were evaluated concerning their growth, regenerability and enzyme activity (POX, SOD, CAT) as a response to the preservation conditions in the incipient phase of the initiation of the in vitro collection. The two species considered in this study showed a good in vitro behavior when using the mannitol as growth retardant. Numéro de notice : A2009-453 Affiliation des auteurs : non IGN Thématique : BIODIVERSITE/FORET Nature : Article DOI : sans En ligne : http://www.editurasilvica.ro/afr/52/1/11_Holobiuc.pdf Permalink : https://documentation.ensg.eu/index.php?lvl=notice_display&id=73512 [article]