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Generation of Expressed Sequence Tags and development of microsatellite markers for Castanopsis sieboldii var. sieboldii (Fagaceae) / Saneyoshi Ueno in Annals of Forest Science, Vol 66 n° 5 (July - August 2009)  

Public [article]  inAnnals of Forest Science > Vol 66 n° 5 (July - August 2009) . - n° 509 Titre : Generation of Expressed Sequence Tags and development of microsatellite markers for Castanopsis sieboldii var. sieboldii (Fagaceae) Titre original : Generation de marqueurs de séquences exprimées et développement de marqueurs microsatellites pour Castanopsis sieboldii var. sieboldii (Fagaceae) Type de document : Article/Communication Auteurs : Saneyoshi Ueno, Auteur ; Kyoko Aoki, Auteur ; Yoshihiko Tsumura, Auteur Année de publication : 2009 Article en page(s) : n° 509 Note générale : Bibliographie Langues : Anglais (eng) Descripteur : [Vedettes matières IGN] Végétation [Termes IGN] arbre caducifolié [Termes IGN] Castanopsis (genre) [Termes IGN] feuillu [Termes IGN] génétique forestière [Termes IGN] Japon Résumé : (auteur) Castanopsis sieboldii var. sieboldii is an evergreen broadleaved canopy tree that grows on Honshu, Shikoku and Kyushu Islands in Japan. A closely-related, hybridizing, congener species, C. cuspidata var. cuspidata, has different leaf epidermis structure and different seed nuts morphology compared to C. sieboldii var. sieboldii. Furthermore, the habitats in which the two species grow often indicate different water requirements. Analysis of genetic diversity, using transcribed sequences, will provide a sound basis for the management of this species, allowing successful reforestation, incorporating phylogeographic conservation. In order to obtain transcripts sequences for the species and to develop transcript-based markers, we constructed and analyzed a cDNA library, surveyed microsatellite sequences within it and designed PCR primers for the microsatellites. The cDNA library was constructed using tissue from the inner bark of C. sieboldii var. sieboldii; 3354 Expressed Sequence Tags (ESTs) were identified. We constructed 2417 putative unigenes and assigned putative functions to 1856 of them. Three hundred and fourteen microsatellites were found within the putative unigenes and 16 EST-SSR (Simple Sequence Repeat) markers were developed. The EST-SSR markers developpe in this study should facilitate future analysis of the genetic diversity of this and related species. Numéro de notice : A2009-643 Affiliation des auteurs : non IGN Thématique : FORET Nature : Article nature-HAL : ArtAvecCL-RevueIntern DOI : 10.1051/forest/2009037 Date de publication en ligne : 09/07/2009 En ligne : https://www.afs-journal.org/articles/forest/full_html/2009/05/f08206/f08206.html Format de la ressource électronique : url article Permalink : https://documentation.ensg.eu/index.php?lvl=notice_display&id=72467 [article]

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Code-barres	Cote	Support	Localisation	Section	Disponibilité
IFN-001-P000455	PER	Revue	Nogent-sur-Vernisson	Salle périodiques	Exclu du prêt