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Biochemical peculiarity of in vitro morphogenesis under conservation strategy of Ruscus aculeatus L. / Cristian Banciu in Annals of forest research, vol 52 n° 1 (January 2009)    

Public [article]  inAnnals of forest research > vol 52 n° 1 (January 2009) . - p. 109-115 Titre : Biochemical peculiarity of in vitro morphogenesis under conservation strategy of Ruscus aculeatus L. Type de document : Article/Communication Auteurs : Cristian Banciu, Auteur ; Monica Elena Mitoi, Auteur ; Aurelia Brezeanu, Auteur Année de publication : 2009 Article en page(s) : p. 109-115 Note générale : Bibliogr. Langues : Anglais (eng) Descripteur : [Termes IGN] conservation de la flore [Termes IGN] microbouturage [Termes IGN] morphogenèse [Termes IGN] Ruscus aculeatus Résumé : (Auteur) "The present study is part of the actual concerns in biodiversity conservation of endangered angyosperm species from the Comana Natural Park. Ruscus aculeatus L. species is protected both at national and European level (trough Habitats Directive of EU and Bern Convention). The aspects of in vitro morphogenesis through all the stages from inoculation, multiplication to rooting and acclimatization have been studied. In order to long term conservation and multiplication or to exploit somaclonal variation induced by in vitro technique is required the identification of some biochemical or molecular markers for fast evaluation of regenerants. In this respect, genetic stability or variability of Ruscus aculeatus plants collected from three populations and regenerants obtained in vitro culture was estimated by electrophoretical methods. Therefore, zymograms of different enzymes as esterase, acid and alkaline phosphatase, glutamate-oxaloacetate transferase, malate dehydrogenase and peroxidase were analyzed. The expression of esterases, phosphatases, malate dehydrogenases displayed changes in correlation with growth condition, while the peroxidases pattern was more stabile in natural population as well as in vitro regenerated plantlet." Numéro de notice : IFN_5799 Affiliation des auteurs : non IGN Thématique : BIODIVERSITE Nature : Article DOI : sans En ligne : http://www.editurasilvica.ro/afr/52/1/10_Banciu.pdf Permalink : https://documentation.ensg.eu/index.php?lvl=notice_display&id=73511 [article]

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